Here are some more images from our PLoS ONE paper in 2012, showing HeLa cells stably expressing a Chromobody against endogenous PCNA. This marks sites of active DNA replication during S phase. For more information please see Burgess, A., Lorca, T., and Castro, A. (2012) Quantitative live imaging of endogenous DNA replication in mammalian cells. PLoS ONE 7, e45726journals.plos.org/plosone/article?id=10.1371/journal.pone.0045726Cast: Cell Division LabTags: Chromotek, Chromobody, mitosis, cell cycle, cell division, timelapse, PCNA, S phase, DNA replication, GFP, Imaris and HeLa